State Key Laboratory of Chemical Resource Engineering, Ministry of Education, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing 100029 China.
Langmuir. 2011 Sep 20;27(18):11575-81. doi: 10.1021/la202101q. Epub 2011 Aug 18.
Polycaprolactone (PCL) has been widely adopted as a scaffold biomaterial, but further improvement of the hemocompatibility of a PCL film surface is still needed for wide biomedical applications. In this work, the PCL film surface was functionalized with zwitterionic poly(3-dimethyl(methacryloyloxyethyl) ammonium propane sulfonate) (P(DMAPS)) brushes via surface-initiated atom transfer radical polymerization (ATRP) for enhancing hemocompatibility. Kinetics study revealed an approximately linear increase in graft yield of the functional P(DMAPS) brushes with polymerization time. The blood compatibilities of the modified PCL film surfaces were studied by platelet adhesion tests of platelet-rich plasma and human whole blood, hemolysis assay, and plasma recalcification time (PRT) assay. The improvement of hemocompatibility is dependent on the coverage of the grafted P(DMAPS) brushes on the PCL film. Lower or no platelet and blood cell adhesion was observed on the P(DMAPS)-grafted film surfaces. The P(DMAPS) grafting can further decrease hemolysis and enhance the PRT of the PCL surface. With the versatility of surface-initiated ATRP and the excellent hemocompatibility of zwitterionic polymer brushes, PCL films with desirable blood properties can be readily tailored to cater to various biomedical applications.
聚己内酯（PCL）已被广泛用作支架生物材料，但为了广泛的生物医学应用，仍需要进一步改善 PCL 薄膜表面的血液相容性。在这项工作中，通过表面引发原子转移自由基聚合（ATRP），将两性离子聚（3-二甲基（甲基丙烯酰氧乙基）铵丙烷磺酸盐）（P（DMAPS））刷接枝到 PCL 薄膜表面，以提高血液相容性。动力学研究表明，接枝功能 P（DMAPS）刷的接枝率随聚合时间呈近似线性增加。通过富血小板血浆和人全血血小板黏附试验、溶血试验和血浆再钙化时间（PRT）试验研究了改性 PCL 薄膜表面的血液相容性。血液相容性的改善取决于接枝在 PCL 薄膜上的接枝 P（DMAPS）刷的覆盖率。在接枝 P（DMAPS）的薄膜表面观察到血小板和血细胞黏附较低或无黏附。接枝 P（DMAPS）可以进一步降低溶血并提高 PCL 表面的 PRT。通过表面引发 ATRP 的多功能性和两性离子聚合物刷的优异血液相容性，可以很容易地定制具有理想血液特性的 PCL 薄膜，以满足各种生物医学应用的需求。
