吖啶橙（AO）-罗丹明B（RB）二聚体能量转移体系在DNA测定中的应用及机制研究

[Studies on application and mechanism of energy transfer system of acridine orange (AO)-rhodamine B (RB) dimer in the determination of DNA].

作者信息

Gao Feng, Zhu Chang-qing, Wang Lun

机构信息

College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):85-8.

PMID:15768984

Abstract

Not only dye monomer is the form of energy transfer but also dye dimer is the effective form of energy transfer. In this work, the energy transfer system of acridine orange (AO)-rhodamine B (RB) dimer was built for the determination of DNA as fluorescence probe. At the same time, the mechanism was also considered. Under the optimum experimental conditions, the linear range of this assay was 0.33-1.33 mg x L(-1) for fish sperm DNA and 0.33-3.33 mg x L(-1) for calf thymus DNA, the detection limit was 1.63 x 10(-3) mg x L(-1) for fish sperm DNA and 1.52 x 10(-3) mg x L(-1) for calf thymus DNA, respectively. The RSD for the determination of 1.00 mg x L(-1) DNA was 2.40% for fsDNA and 2.00% for ctDNA, respectively. The method was used for the determination of DNA in synthetic samples with satisfied results.

摘要

不仅染料单体是能量转移的形式，染料二聚体也是有效的能量转移形式。在本工作中，构建了吖啶橙（AO）-罗丹明B（RB）二聚体的能量转移体系作为荧光探针用于DNA的测定。同时，也对其机理进行了探讨。在最佳实验条件下，该测定方法对鱼精DNA的线性范围为0.33 - 1.33 mg·L⁻¹，对小牛胸腺DNA的线性范围为0.33 - 3.33 mg·L⁻¹，对鱼精DNA的检测限为1.63×10⁻³ mg·L⁻¹，对小牛胸腺DNA的检测限为1.52×10⁻³ mg·L⁻¹。测定1.00 mg·L⁻¹ DNA时，鱼精DNA的相对标准偏差为2.40%，小牛胸腺DNA的相对标准偏差为2.00%。该方法用于合成样品中DNA的测定，结果令人满意。