[用吖啶橙共振光散射技术测定脱氧核糖核酸]

[Determination of deoxyribonucleic acid with acridine orange by resonance light scattering technique].

作者信息

Wan Xin-Jun, Liu Jin-Shui, Wang Lun

机构信息

Department of Chemistry, Chaohu College, Chaohu 238000, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2005 May;25(5):754-6.

PMID:16128081

Abstract

The resonance light scattering (RLS) spectra of acridine orange with deoxyribonucleic acid have been studied. The RLS of acridine orange is greatly enhanced by deoxyribonucleic acid in pH 11. There is a resonance light scattering peak at 324 nm. Under optimal conditions, the linear ranges of the calibration curves are 7.5 x 10(-8) -9.8 x 10(-6) g x mL(-1) for Calf thymus DNA (ct DNA), 3.1 x 10(-8) -7.3 x 10(-6) g x mL(-1) for Fish sperm DNA (fs DNA) with detection limits 20.8 ng x mL(-1) for ct DNA and 20.5 ng x mL(-1) for fs DNA. This method is simple, selectivity and sensitivity has been applied to the determination of deoxyribonucleic acid in mixed samples with satisfactory results.

摘要

研究了吖啶橙与脱氧核糖核酸的共振光散射（RLS）光谱。在pH 11条件下，脱氧核糖核酸能极大地增强吖啶橙的共振光散射。在324nm处有一个共振光散射峰。在最佳条件下，小牛胸腺DNA（ct DNA）校准曲线的线性范围为7.5×10⁻⁸ - 9.8×10⁻⁶ g·mL⁻¹，鱼精DNA（fs DNA）校准曲线的线性范围为3.1×10⁻⁸ - 7.3×10⁻⁶ g·mL⁻¹，ct DNA的检测限为20.8 ng·mL⁻¹，fs DNA的检测限为20.5 ng·mL⁻¹。该方法简便，具有选择性和灵敏度，已应用于混合样品中脱氧核糖核酸的测定，结果令人满意。