Wang Le-yu, Zhao Chang-qing, Zhu Chang-qing, Wang Lun
College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):98-101.
In this work, nano-ZnS has been successfully prepared. The nano-ZnS has also been modified with sodium mercaptoacetic acid. The functionalized nanoparticles are water-soluble and biocompatible. All the nanoparticles have been characterized by IR spectra, UV spectra, fluorescence spectra and TEM images. In comparison with single organic fluorophore, the nanoparticles probes are brighter and more photostable, and do not suffer from blinking. The nanoparticles have a narrow, tunable, symmetric emission spectrum and a broad, continuous excitation spectrum. They are also photochemically stable. Effects of proteins and nucleic acids on the UV spectra and fluorescence spectra of the functionalized ZnS have also been studied. The intensities of UV spectra and fluorescence spectra of the functionalized ZnS are enhanced by proteins, and, however, are quenched by nucleic acids. The functionalized colloidal solutions prepared are hopeful of use as fluorescence probes in biological staining and diagnostics.
在本工作中，已成功制备出纳米硫化锌。纳米硫化锌也已用巯基乙酸钠进行了改性。功能化的纳米颗粒具有水溶性和生物相容性。所有纳米颗粒均通过红外光谱、紫外光谱、荧光光谱和透射电镜图像进行了表征。与单一有机荧光团相比，纳米颗粒探针更亮且光稳定性更高，并且不存在闪烁现象。纳米颗粒具有窄的、可调谐的、对称的发射光谱和宽的、连续的激发光谱。它们在光化学上也很稳定。还研究了蛋白质和核酸对功能化硫化锌的紫外光谱和荧光光谱的影响。蛋白质会增强功能化硫化锌的紫外光谱和荧光光谱强度，然而，核酸会使其猝灭。所制备的功能化胶体溶液有望用作生物染色和诊断中的荧光探针。
