[小檗碱与人类血清白蛋白的相互作用]

[The interaction of berberine and human serum albumin].

作者信息

Zhao Chang-chun, Zheng Wei-fa, Li Meng-qiu

机构信息

Department of Chemistry, Xuzhou Normal University, Xuzhou 221009, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):111-3.

PMID:15768991

Abstract

The interaction of berberine and Human Serum Albumin (HSA) has been studied by fluorescence and UV-Vis absorption spectroscopy. With fluorescence quenching method, the binding constant K was found to be 1.168 x 10(5) L x mol(-1) and the number of binding site n was 5.26. The binding distance (R = 3.44 nm) and energy transfer efficiency (E = 0.303) were also obtained according to Förster's non-radiation energy transfer theory. The energy transfer path between tyrosine residual and tryptophan residual was cut by berberine in the site of HSA and weak fluorescence berberine-HSA complex was also observed.

摘要

通过荧光光谱和紫外可见吸收光谱研究了黄连素与人类血清白蛋白（HSA）的相互作用。采用荧光猝灭法，测得结合常数K为1.168×10⁵ L·mol⁻¹，结合位点数n为5.26。根据Förster非辐射能量转移理论，还得到了结合距离（R = 3.44 nm）和能量转移效率（E = 0.303）。黄连素在HSA位点切断了酪氨酸残基和色氨酸残基之间的能量转移路径，并且还观察到了弱荧光的黄连素-HSA复合物。