Kim Moon Suk, Seo Kwang Su, Khang Gilson, Lee Hai Bang
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea.
Bioconjug Chem. 2005 Mar-Apr;16(2):245-9. doi: 10.1021/bc049860l.
A novel biotinylated gradient surface was designed to bind a streptavidin-FITC. The gradient polyethylene (PE) surface was prepared by a corona treatment with a power increase along the PE length, and successively, biotinylated gradient PE surface was prepared by the reaction of polyethylenimine (PEI) and biotin. Surface properties of the gradient PE surface changed according to PE length and chemical modification. The biotinylated gradient PE surface provided a continuous gradient of streptavidin-FITC through binding of avidin-biotin. The luminosity of fluorescence that corresponds to a streptavidin-bound FITC on the biotinylated gradient PE surface increased gradually on the surface along the PE length.
设计了一种新型生物素化梯度表面以结合链霉亲和素-异硫氰酸荧光素（streptavidin-FITC）。通过沿聚乙烯（PE）长度增加功率的电晕处理制备梯度聚乙烯（PE）表面，随后，通过聚乙烯亚胺（PEI）与生物素的反应制备生物素化梯度PE表面。梯度PE表面的表面性质根据PE长度和化学修饰而变化。生物素化梯度PE表面通过抗生物素蛋白-生物素的结合提供了链霉亲和素-异硫氰酸荧光素的连续梯度。在生物素化梯度PE表面上，与结合链霉亲和素的异硫氰酸荧光素相对应的荧光亮度沿PE长度在表面上逐渐增加。
