Kim Moon Suk, Seo Kwang Su, Khang Gilson, Lee Hai Bang
Nanobiomaterials Laboratory, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea.
Langmuir. 2005 Apr 26;21(9):4066-70. doi: 10.1021/la046868a.
A gradient polyethylene (PE) surface was created through corona treatment, in which the corona power increased along the 5 cm length of the PE. The gradient surface was treated with polyethyleneimine and then biotin. Fluorescein-conjugated streptavidin (SAV) caged within 5-carboxymethoxy-2-nitrobenzyl (CMNB) adsorbed onto the biotinylated gradient PE surface following molecular recognition principles. Photoirradiation decomposed the CMNB cage and allowed the fluorescein-conjugated SAV to fluoresce, the intensity of which increased gradually along the PE surface.
通过电晕处理创建了一个梯度聚乙烯（PE）表面，其中电晕功率沿PE的5厘米长度增加。对该梯度表面进行聚乙烯亚胺处理，然后再用生物素处理。根据分子识别原理，将包裹在5-羧基甲氧基-2-硝基苄基（CMNB）中的荧光素共轭链霉亲和素（SAV）吸附到生物素化的梯度PE表面上。光照射分解了CMNB笼，使荧光素共轭SAV发出荧光，其强度沿PE表面逐渐增加。
