Wang S B
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1992 Nov;13(6):439-41.
DNA content and cell cycle of irradiated HeLa S3 cells in the exponential phase was measured by image analysis and flow cytometric analysis. The HeLa S3 cell phases had undergone remarkable changes after irradiation. There was a dose dependent radiation effect. As the radiation dose increased, the number of G0 + G1 and S phase cells were reduced and G2 + M phase delay occurred. The number of G2 + M phase in HeLa S3 cells after 6-8 Gy was increased from 22% to 52%-61%. Meanwhile, the DNA content of HeLa S3 cells was increased from 6.72 to 9.08-10.0 Au, the results of these two assays were compatible. Most of the delay of G2 + M phase appeared 24 hours after irradiation. The concentration of HeLa S3 cells used varied from 32 x 10(4)/ml to 147 x 10(4)/ml in the experiments. No relationship was observed in the changes of phase and cell concentrations.
通过图像分析和流式细胞术分析，测定指数生长期受辐照的HeLa S3细胞的DNA含量和细胞周期。辐照后HeLa S3细胞的各时相发生了显著变化。存在剂量依赖性辐射效应。随着辐射剂量增加，G0 + G1期和S期细胞数量减少，G2 + M期出现延迟。6 - 8 Gy辐照后HeLa S3细胞的G2 + M期细胞数量从22%增加到52% - 61%。同时，HeLa S3细胞的DNA含量从6.72增加到9.08 - 10.0 Au，这两种检测结果相符。G2 + M期的延迟大多出现在辐照后24小时。实验中使用的HeLa S3细胞浓度在32×10⁴/ml至147×10⁴/ml之间变化。未观察到细胞时相变化与细胞浓度之间存在关联。
