Murata T, Akagi K, Uda M, Kimura H, Nasu R, Tanaka Y
Department of Radiology, Kansai Medical University, 10-15 Fumizonocho, Moriguchi-city, Osaka, Japan.
Int J Oncol. 1998 Jan;12(1):171-4.
Flow cytometry instantaneously determines the percentages of cells in various cell cycle phases to rapidly evaluate effects of irradiation and anti-cancer drugs. We studied these using growth curve analysis and 5-bromodeoxyuridine propidium iodine (BrdU)-(PI) double staining. With conventional DNA histogram methods, determination of S phase fraction was difficult because of overlapping DNA content between G1 and early S phases and between late S and G2 phases. Double staining directly differentiated G1, S, and G2 + M phases. By double staining, rapid transition from G1 to S occurred within 4 h after irradiation or after the drug treatments, and initial G1 arrest induced by irradiation was confirmed for the first time.
流式细胞术可即时测定处于不同细胞周期阶段的细胞百分比，从而快速评估辐射和抗癌药物的效果。我们使用生长曲线分析和5-溴脱氧尿苷-碘化丙啶（BrdU）-（PI）双重染色法对此进行了研究。采用传统的DNA直方图方法时，由于G1期与S期早期之间以及S期晚期与G2期之间的DNA含量重叠，难以确定S期细胞比例。双重染色可直接区分G1期、S期和G2 + M期。通过双重染色发现，辐射或药物处理后4小时内，细胞从G1期快速过渡到S期，并且首次证实了辐射诱导的初始G1期停滞。
