Kilic Ayben, Kollmannsberger Hubert, Nitz Siegfried
Faculty of Forestry, Zonguldak Karaelmas University, 74100 Bartin, Turkey.
J Agric Food Chem. 2005 Mar 23;53(6):2231-5. doi: 10.1021/jf040373+.
Glycosidically bound volatile compounds in different parts (leaves and buds) of Laurus nobilis L. were investigated. After isolation of extracts obtained by Amberlite XAD-2 adsorption and methanol elution, glycosides were analyzed after enzymatic hydrolysis by GC-MS or directly after trifluoroacetyl (TFA) derivatization by GC-MS in EI and NCI mode. In the leaves most of the glycosidically bound volatiles occur as beta-D-glucopyranosides. Among the disaccharides, primeverosides are predominant; smaller amounts of alpha-L-arabinofuranosyl-beta-D-glucopyranosides, rutinosides, and vicianocides could also be identified. Major aglycons comprised benzyl alcohol, some linalool-diols, 2-hydroxy-1,8-cineole and its derivatives such as 2,3-dehydro-1,8-cineole, sobrerols, and menthadien-8-ols. Among the identified nor-carotenoids, 3-oxo-alpha-ionol, the corresponding 7,8-dihydro derivative, and vomifoliol are predominant in leaves. 3-Hydroxy-beta-damascone and 3-hydroxy-7,8-didehydro-beta-ionol, precursors of the sensorially active damascenone, were identified only in the buds.
对月桂叶不同部位（叶片和芽）中糖苷结合的挥发性化合物进行了研究。通过Amberlite XAD - 2吸附和甲醇洗脱获得提取物后，糖苷在酶促水解后通过气相色谱 - 质谱联用仪（GC - MS）进行分析，或者在三氟乙酰（TFA）衍生化后直接通过气相色谱 - 质谱联用仪在电子轰击（EI）和负离子化学电离（NCI）模式下进行分析。在叶片中，大多数糖苷结合的挥发性化合物以β - D - 吡喃葡萄糖苷的形式存在。在二糖中，樱草糖苷占主导；还可鉴定出少量的α - L - 阿拉伯呋喃糖基 - β - D - 吡喃葡萄糖苷、芸香糖苷和蚕豆糖苷。主要的苷元包括苯甲醇、一些芳樟醇二醇、2 - 羟基 - 1,8 - 桉叶素及其衍生物，如2,3 - 脱氢 - 1,8 - 桉叶素、异松油醇和薄荷二烯 - 8 - 醇。在已鉴定的去甲类胡萝卜素中，3 - 氧代 - α - 紫罗兰醇、相应的7,8 - 二氢衍生物和vomifoliol在叶片中占主导。3 - 羟基 - β - 大马酮和3 - 羟基 - 7,8 - 二脱氢 - β - 紫罗兰醇，即具有感官活性的大马酮的前体，仅在芽中被鉴定出。
