Palumbo Antonio, Casanova Livia Marques, Corrêa Maria Fernanda Paresqui, Da Costa Nathalia Meireles, Nasciutti Luiz Eurico, Costa Sônia Soares
Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
Instituto de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
Evid Based Complement Alternat Med. 2019 Jan 2;2019:6340757. doi: 10.1155/2019/6340757. eCollection 2019.
Benign Prostatic Hyperplasia (BPH) affects mainly older men. It is estimated to affect 50% of 51-60-year-old men and 70% of 61-70-year-old men. BPH is a nonmalignant proliferation of epithelial and stromal cells of the prostate gland regions. Despite the use of conventional pharmacological therapy, herbal medicines are used in BPH therapy, and several mechanisms of action have been suggested based on their complex chemical composition. Considering the ethnomedicinal uses of (KGB), we evaluated the inhibitory effects on the proliferation of stromal cells from primary benign prostatic hyperplasia (BPH) of four different aqueous extracts from this plant: underground parts from specimens in flower (T1 treatment), leaves from specimens in flower (T2 treatment), and flowers (T3 treatment) and leaves from specimens not in flower (T4 treatment). T1, T2, T3, and T4 treatments at 250 g/ml for 72 hours inhibited BPH cells by 56.7%, 29.2%, 39.4%, and 13.5%, respectively, showing that the KGB underground parts extract (T1 treatment) was the most active. Our findings show that the extract of the KGB underground parts (150 and 250 g/ml) stimulates important changes in the BPH cells, modulating crucial processes such as proliferation, viability, and apoptosis. HPLC-DAD-MS/MS analysis provided a tentative identification of glycosylated syringic acid derivatives, glycosylated forms of volatile compounds, and lignans in this extract. Finally, these results suggest that there is a potential therapeutic use for KGB in BPH, which could improve the clinical management of the disease.
良性前列腺增生（BPH）主要影响老年男性。据估计，51至60岁的男性中有50%受其影响，61至70岁的男性中有70%受其影响。BPH是前列腺区域上皮和基质细胞的非恶性增殖。尽管使用了传统的药物治疗，但草药也被用于BPH治疗，基于其复杂的化学成分提出了几种作用机制。考虑到（KGB）的民族药用用途，我们评估了该植物四种不同水提取物对原发性良性前列腺增生（BPH）基质细胞增殖的抑制作用：花期标本的地下部分（T1处理）、花期标本的叶子（T2处理）、花朵（T3处理）和非花期标本的叶子（T4处理）。在250微克/毫升的浓度下处理72小时，T1、T2、T3和T4处理分别使BPH细胞减少了56.7%、29.2%、39.4%和13.5%，表明KGB地下部分提取物（T1处理）活性最高。我们的研究结果表明，KGB地下部分提取物（150和250微克/毫升）可刺激BPH细胞发生重要变化，调节增殖、活力和凋亡等关键过程。HPLC-DAD-MS/MS分析初步鉴定了该提取物中的糖基化丁香酸衍生物、挥发性化合物的糖基化形式和木脂素。最后，这些结果表明KGB在BPH治疗中具有潜在的治疗用途，这可能会改善该疾病的临床管理。
