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月桂叶、芽、花和果实中的挥发性成分及关键气味物质

Volatile constituents and key odorants in leaves, buds, flowers, and fruits of Laurus nobilis L.

作者信息

Kilic Ayben, Hafizoglu Harzemsah, Kollmannsberger Hubert, Nitz Siegfried

机构信息

Faculty of Forestry, Zonguldak Karaelmas University, 74100 Bartin, Turkey.

出版信息

J Agric Food Chem. 2004 Mar 24;52(6):1601-6. doi: 10.1021/jf0306237.

Abstract

The volatiles of fresh leaves, buds, flowers, and fruits from bay (Laurus nolilis L.) were isolated by solvent extraction and analyzed by capillary gas chromatography-mass spectrometry. Their odor quality was characterized by gas chomatography-olfactometry-mass spectrometry (HRGC-O-MS) and aroma extract dilution analysis (AEDA). In fresh bay leaves 1,8-cineole was the major component, together with alpha-terpinyl acetate, sabinene, alpha-pinene, beta-pinene, beta-elemene, alpha-terpineol, linalool, and eugenol. Besides 1,8-cineole and the pinenes, the main components in flowers were alpha-eudesmol, beta-elemene, and beta-caryophyllene, in fruits (E)-beta-ocimene and biclyclogermacrene, and in buds (E)-beta-ocimene and germacrene D. The aliphatic ocimenes and farnesenes were absent in leaves. By using HRGC-O-MS 21 odor compounds were identified in fresh leaves. Application of AEDA revealed (Z)-3-hexenal (fresh green), 1,8-cineole (eucalyptus), linalool (flowery), eugenol (clove), (E)-isoeugenol (flowery), and an unidentified compound (black pepper) with the highest flavor dilution factors. Differences between buds, flowers, fruits, and leaves with regard to the identified odor compounds are presented.

摘要

采用溶剂萃取法分离月桂(Laurus nolilis L.)鲜叶、芽、花和果实中的挥发性成分,并用毛细管气相色谱 - 质谱联用仪进行分析。通过气相色谱 - 嗅觉测定 - 质谱联用仪(HRGC - O - MS)和香气提取物稀释分析(AEDA)对其气味特征进行表征。鲜月桂叶中,1,8 - 桉叶素是主要成分,还有乙酸α - 萜品酯、桧烯、α - 蒎烯、β - 蒎烯、β - 榄香烯、α - 松油醇、芳樟醇和丁香酚。除了1,8 - 桉叶素和蒎烯外,花中的主要成分是α - 桉叶醇、β - 榄香烯和β - 石竹烯,果实中的主要成分是(E)-β - 罗勒烯和双环大根香叶烯,芽中的主要成分是(E)-β - 罗勒烯和大根香叶烯D。叶中不存在脂肪族罗勒烯和法呢烯。通过HRGC - O - MS在鲜叶中鉴定出21种气味化合物。AEDA分析表明,(Z)-3 - 己烯醛(新鲜青香)、1,8 - 桉叶素(桉叶香)、芳樟醇(花香)、丁香酚(丁香香)、(E)-异丁香酚(花香)和一种未鉴定的化合物(黑胡椒香)具有最高的风味稀释因子。文中呈现了芽、花、果实和叶在已鉴定出的气味化合物方面的差异。

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