[四川彝族人群人类免疫缺陷病毒共受体CCR5Delta32和CCR2 - 64I等位基因的遗传多态性]

[Genetic polymorphism of human immunodeficiency virus coreceptor CCR5Delta32 and CCR2-64I alleles in Chinese Yi Ethnic group in Sichuan].

作者信息

Deng Xiao-Ling, Hong Kun-Xue, Chen Jian-Ping, Ruan Yu-Hua, Xu Ming-Yan, Qin Guang-Ming, Li Ke, Xing Hui, Shao Yi-Ming

机构信息

National Center for Sexually Transmitted Disease and Acquired Immune Deficiency Syndrome Control and Prevention, Chinese Center for Disease Centrol and Prevention, Beijing 100050, China.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 2004 Dec;25(12):1050-3.

PMID:15769362

Abstract

OBJECTIVE

To explore genetic polymorphisms CCR5 of HIV coreceptor and CCR2 in Chinese Yi Ethnic group in Sichuan.

METHODS

Genomic DNA samples were obtained from 119 healthy individuals and 88 HIV-1 infected individuals of Chinese Yi Ethnic group in Sichuan. Polymerase chain reaction (PCR), cloning and gene sequencing techniques were employed to identify the genotype of CCR5Delta32; PCR-restriction fragment length polymorphism (RFLP) and gene sequencing were employed to identify the CCR2-64I alleles.

RESULTS

At CCR5 locus, 2 heterozygotes (CCR5-wt/Delta32) and none homozygote (CCR5-Delta32/Delta32) were observed in 119 healthy individuals, allelic frequency of CCR5-Delta32 was 0.84%; No mutant was found in 88 HIV-1 infected individuals. At CCR2 locus, 26 heterozygotes (CCR2-64V/64I) and two homozygotes (CCR2-64I/64I) were observed in healthy individuals but the allelic frequency CCR2-64I was 12.61%. Among infected individuals, 12 heterozygotes (CCR2-64V/64I) and 7 homozygotes (CCR2-64I/64I) were observed and the allelic frequency CCR2-64I was 13.27%. Statistical analysis revealed that the differences of both loci between healthy and infected individuals were insignificant. Both loci were consistent with the Hardy-Weinberg equilibrium in the two different groups.

CONCLUSION

The polymorphism of CCR5Delta32 and CCR2-64I alleles from Chinese Yi Ethnic group was detected which was of significance for the evaluation of genetic resistance to HIV-1 infection in Chinese population.

摘要

目的

探讨四川彝族人群中HIV共受体CCR5及CCR2的基因多态性。

方法

采集119名四川彝族健康个体及88名HIV-1感染者的基因组DNA样本。采用聚合酶链反应（PCR）、克隆及基因测序技术鉴定CCR5Delta32基因型；采用PCR-限制性片段长度多态性（RFLP）及基因测序鉴定CCR2-64I等位基因。

结果

在CCR5位点，119名健康个体中观察到2例杂合子（CCR5-wt/Delta32），无纯合子（CCR5-Delta32/Delta32），CCR5-Delta32等位基因频率为0.84%；88名HIV-1感染者中未发现突变。在CCR2位点，健康个体中观察到26例杂合子（CCR2-64V/64I）及2例纯合子（CCR2-64I/64I），CCR2-64I等位基因频率为12.61%。感染者中观察到12例杂合子（CCR2-64V/64I）及7例纯合子（CCR2-64I/64I），CCR2-64I等位基因频率为13.27%。统计学分析显示，健康个体与感染者在两个位点的差异均无统计学意义。两个位点在两组不同人群中均符合Hardy-Weinberg平衡。