Fernandez Philippe, Deguette Audrey, Pothuaud Laurent, Belleannée Geneviève, Coste Pierre, Bordenave Laurence
INSERM-U577, Université Victor Segalen Bordeaux 2, 146, Rue Leo Saignat, 33320 Bordeaux, France.
Biomaterials. 2005 Aug;26(24):5042-7. doi: 10.1016/j.biomaterials.2005.01.011.
Over the past two decades, in vitro autologous endothelial cell (EC) coverage of expanded polytetrafluoroethylene (ePTFE) graft has been developed and clinically applied with success in infrainguinal bypasses. Before endothelialization, the luminal surface of the graft has to be coated with a currently used fibrinolytically inhibited fibrin glue. The aim of this work is to validate the precoating of the ePTFE (4 mm ID) ringed graft with fibrin. Twenty cm-long grafts were precoated with fibrin glue (2 operators) then fixed for microscopy investigations. Grafts were sliced into 3 regions. Thickness analysis was evaluated by image processing. Three grafts have been tested for endothelialization and observed at days 3, 8. Cell-free coated ePTFE were imaged using high-frequency ultrasound modality. Whatever the examined segment an overall homogeneous covering protein is shown. Fibrin thickness after image processing is 8.5+/-0.25 and 4.1+/-0.4 microm for two operators (P < .001). We have evaluated reproducibility and inter- and intra-variability of the operator, assessed quality controls and quality assurance all along the prosthesis and finally endothelialization and subsequent behaviour under shear stress conditions.
在过去二十年中，体外自体内皮细胞（EC）覆盖的膨体聚四氟乙烯（ePTFE）移植物已被研发出来，并在临床应用于股下旁路移植手术且取得成功。在内皮化之前，移植物的管腔表面必须用目前使用的具有纤维蛋白溶解抑制作用的纤维蛋白胶进行涂层处理。这项工作的目的是验证用纤维蛋白对内径4毫米的带环ePTFE移植物进行预涂层处理的效果。将20厘米长的移植物用纤维蛋白胶进行预涂层处理（由2名操作人员操作），然后固定用于显微镜检查。将移植物切成3个区域。通过图像处理评估厚度分析。对3个移植物进行内皮化测试，并在第3天、第8天进行观察。使用高频超声模式对无细胞涂层的ePTFE进行成像。无论检查的是哪个节段，都显示出整体均匀的覆盖蛋白。两名操作人员图像处理后的纤维蛋白厚度分别为8.5±0.25微米和4.1±0.4微米（P <.001）。我们评估了操作人员的可重复性以及操作者间和操作者内的变异性，在整个假体上评估了质量控制和质量保证，最后评估了内皮化以及随后在剪切应力条件下的行为。
