Naik Nisarga, Kumar Vivek, Chaikof Elliot L, Allen Mark G
,School of Electrical and Computer Engineering of Georgia Institute of Technology, Atlanta, GA 30332, USA.
Annu Int Conf IEEE Eng Med Biol Soc. 2011;2011:290-3. doi: 10.1109/IEMBS.2011.6090076.
The endothelialization of an engineered microvascular network is constrained by the mass transport of the endothelial cells through high length-to-depth (l/d) aspect ratio microchannels. This paper presents a deformable, reentrant microvascular scaffold as a microelectromechanical systems (MEMS)-assisted approach for spatially homogeneous endothelial cell seeding of high l/d (>200) aspect ratio microvasculature. Nickel electroplating and micromolding were employed for the fabrication of the polydimethylsiloxane (PDMS) reentrant microvascular scaffold. A 'stretch--seed--seal' ('3S') operation was implemented for uniform incorporation of endothelial cells on the luminal surface of the elastomeric constructs. Confocal microscopy was utilized to establish the uniformity of endothelialization and to demonstrate the feasibility of this strategy.
工程化微血管网络的内皮化受到内皮细胞通过高长径比（l/d）微通道的传质限制。本文提出了一种可变形的、凹入式微血管支架，作为一种微机电系统（MEMS）辅助方法，用于高l/d（>200）长径比微脉管系统的空间均匀内皮细胞接种。采用镍电镀和微成型工艺制备聚二甲基硅氧烷（PDMS）凹入式微血管支架。实施了“拉伸-接种-密封”（“3S”）操作，以使内皮细胞均匀地掺入弹性体构建体的管腔表面。利用共聚焦显微镜确定内皮化的均匀性，并证明该策略的可行性。
