Kilic Abdullah, Baysallar Mehmet, Bahar Gul, Kucukkaraaslan Ayten, Cilli Feriha, Doganci Levent
Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy and School of Medicine, 06018, Ankara, Turkey 2Department of Microbiology, Social Security Education Hospital, Ankara, Turkey 3Department of Microbiology and Clinical Microbiology, Ege University Medical Faculty, Izmir, Turkey.
J Med Microbiol. 2005 Apr;54(Pt 4):347-350. doi: 10.1099/jmm.0.45789-0.
The aim of this study was to evaluate the performance of the EVIGENE VRE Detection kit and compare it with PCR, considered the gold standard for detection of vancomycin-resistant enterococci (VRE). The correlation between the MIC values of vancomycin and teicoplanin using the epsilon test was also determined. In the EVIGENE VRE Detection kit, DNA probes specific for bacterial target DNA sequences are bound to microwell plates. A hundred and ten VRE (104 Enterococcus faecium and six Enterococcus faecalis) and 45 vancomycin-susceptible E. faecium were tested. All VRE strains were found to be positive for the vanA genotype using the EVIGENE VRE Detection kit. All results obtained with the EVIGENE VRE Detection kit were confirmed by PCR. MIC results for the strains also correlated highly with the PCR and kit results. The EVIGENE VRE Detection kit should be used in preference to other methods for detecting resistance genes in all strains, since it is less time-consuming, does not require the handling of hazardous chemicals and has the same specificity as PCR.
本研究的目的是评估EVIGENE耐万古霉素肠球菌（VRE）检测试剂盒的性能，并将其与被视为检测耐万古霉素肠球菌（VRE）金标准的聚合酶链反应（PCR）进行比较。还确定了使用埃普西隆试验时万古霉素和替考拉宁最低抑菌浓度（MIC）值之间的相关性。在EVIGENE VRE检测试剂盒中，针对细菌靶DNA序列的DNA探针被固定在微孔板上。对110株VRE（104株粪肠球菌和6株屎肠球菌）和45株对万古霉素敏感的粪肠球菌进行了检测。使用EVIGENE VRE检测试剂盒发现所有VRE菌株的vanA基因型均为阳性。EVIGENE VRE检测试剂盒获得的所有结果均通过PCR得到证实。这些菌株的MIC结果也与PCR和试剂盒结果高度相关。EVIGENE VRE检测试剂盒应优先于其他方法用于检测所有菌株中的耐药基因，因为它耗时较少，不需要处理危险化学品，并且与PCR具有相同的特异性。
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