评估AdvanDx VRE EVIGENE检测法用于检测耐万古霉素金黄色葡萄球菌中的vanA基因。
Evaluation of the AdvanDx VRE EVIGENE assay for detection of vanA in vancomycin-resistant Staphylococcus aureus.
作者信息
Fosheim G E, Carey R B, Limbago B M
机构信息
Division of Healthcare Quality Promotion, National Center for Preparedness, Detection, and Control of Infectious Diseases, Centers for Disease Control and Prevention, MS C16, 1600 Clifton Rd., Atlanta, GA 30333, USA.
出版信息
J Clin Microbiol. 2007 May;45(5):1611-3. doi: 10.1128/JCM.02556-06. Epub 2007 Mar 7.
Abstract
AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay demonstrated 100% sensitivity and specificity when analyzed visually and by optical density.
摘要
AdvanDx公司的VRE EVIGENE是一种用于鉴定耐万古霉素肠球菌（VRE）的商业化vanA/vanB DNA杂交检测法，该检测法针对金黄色葡萄球菌中vanA的检测进行了评估。使用金黄色葡萄球菌、VRE以及万古霉素中介和敏感菌株评估了其性能。该检测法在目视分析和光密度分析时均显示出100%的灵敏度和特异性。
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