巴西患乳腺炎奶牛乳汁中念珠菌属的分离
Isolation of Candida spp. from mastitic bovine milk in Brazil.
作者信息
de Casia dos Santos Rita, Marin José Moacir
机构信息
Departamento de Microbiologia da Faculdade de Ciências Agrária e Veterinárias de Jaboticabal, Universidade Estadual Paulista, Brazil.
出版信息
Mycopathologia. 2005 Feb;159(2):251-3. doi: 10.1007/s11046-004-2229-2.
The purpose of this study was to isolate yeast (Candida) from the quarter milk of cow udders from 37 dairy farms in Brazil and to identify the different species involved in mastitis. The samples were collected between October 2002 and February 2003. Two-hundred-and-sixty milk samples from cows with clinical and subclinical mastitis were examined. Milk samples were plated onto Blood agar, Mac Conkey agar and Sabouraud dextrose agar. Forty-five (17.3%) samples were positive for the genus Candida. The Candida species isolated were C. krusei (44.5%), C. rugosa (24.5%), C. albicans (8.9%), C. guilliermondii (8.9%), and others (13.2%). We also isolated Escherichia coli (26.5%), coagulase-positive Staphylococcus (25.0%), Streptococcus spp. (8.1%), Enterobacter spp. (8.1%), and other fungi (8.1%), among others.
本研究的目的是从巴西37个奶牛场的奶牛乳房四分乳中分离酵母菌（念珠菌），并鉴定引起乳腺炎的不同菌种。样本采集于2002年10月至2003年2月之间。对260份患有临床型和亚临床型乳腺炎奶牛的乳样进行了检测。将乳样接种于血琼脂、麦康凯琼脂和沙氏葡萄糖琼脂上。45份（17.3%）样本念珠菌属呈阳性。分离出的念珠菌菌种有克鲁斯念珠菌（44.5%）、皱落念珠菌（24.5%）、白色念珠菌（8.9%）、季也蒙念珠菌（8.9%）以及其他菌种（13.2%）。我们还分离出了大肠杆菌（26.5%）、凝固酶阳性葡萄球菌（25.0%）、链球菌属（8.1%）、肠杆菌属（8.1%）以及其他真菌（8.1%）等。
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