Wimmers K, Lin C L, Tholen E, Jennen D G J, Schellander K, Ponsuksili S
Institute of Animal Breeding and Genetics, University of Bonn, Endenicher Allee 15, D-53115 Bonn, Germany.
Anim Genet. 2005 Apr;36(2):152-5. doi: 10.1111/j.1365-2052.2005.01267.x.
Alpha-actinins (ACTN1 and ACTN4) and gamma-actin (ACTG2), were investigated as candidate genes on the basis of their known functions, for their possible association with sperm concentration, motility, semen volume per ejaculate, plasma droplets rate, abnormal sperm rate and the fertility traits, non-return rate and number of piglets born alive. Polymorphisms were identified in intron 18 (G>A) of the porcine ACTN1 gene and in exon 22 (A>C) of the porcine ACTN4 gene by comparative sequencing of animals from the Pietrain (PI) and Hampshire (HA) breeds. Pietrain (n = 244) and crossbreed PI x HA (n = 112) boars from an artificial insemination boar station were genotyped for the single nucleotide polymorphisms (SNPs) within ACTN1 and ACTN4 as well as for a previously described microsatellite within ACTG2. The study provides evidence for effects of ACTN1 on fertility and of ACTG2 on sperm quality traits, while no indication of impact of ACTN4 on any of these traits was found.
基于已知功能,研究了α - 辅肌动蛋白(ACTN1和ACTN4)和γ - 肌动蛋白(ACTG2)作为候选基因,探讨它们与精子浓度、活力、每次射精的精液量、血浆滴率、异常精子率以及繁殖性状、返情率和产活仔数之间的可能关联。通过对皮特兰(PI)和汉普夏(HA)品种动物进行比较测序,在猪ACTN1基因的第18内含子(G>A)和猪ACTN4基因的第22外显子(A>C)中鉴定出多态性。对来自人工授精种公猪站的皮特兰(n = 244)和杂交种PI×HA(n = 112)公猪进行基因分型,检测ACTN1和ACTN4内的单核苷酸多态性(SNP)以及ACTG2内一个先前描述的微卫星。该研究为ACTN1对繁殖力的影响以及ACTG2对精子质量性状的影响提供了证据,而未发现ACTN4对这些性状有任何影响的迹象。