Shweta Amany, Denton Kate M, Kett Michelle M, Bertram John F, Lambert Gavin W, Anderson Warwick P
Department of Physiology, Monash University, Victoria, Australia.
J Hypertens. 2005 Apr;23(4):851-9. doi: 10.1097/01.hjh.0000163155.29740.d2.
To determine the effects of chronic denervation on renal vascular structure and function in young adult spontaneously hypertensive rats (SHRs).
Unilateral renal denervation (SHRUDx) or sham-operation (SHRS) was performed in SHRs at 6 weeks of age. At 10 weeks, rats were allocated to one of three procedures designed to examine renal vascular structure and function. A further group underwent bilateral renal denervation.
In SHRUDx or SHRS groups, either the kidneys were perfusion-fixed for stereological estimates of artery wall and lumen dimensions or for vascular casting to determine arteriole lumen diameters, or the rats were anaesthetized for estimation of glomerular capillary pressure.
Chronic unilateral renal denervation had no significant effect on the development of hypertension between 6 and 10 weeks of age, as previously reported, but resulted in luminal narrowing of the interlobular artery (denervated group 52 +/- 2 mum, sham-operated group 64 +/- 1 mum; P < 0.01 for interaction between strain and treatment), without alterations in interlobular or arcuate artery wall dimensions. There were no significant effects on either afferent or efferent arteriole lumen diameters. Estimated glomerular capillary pressure was significantly lower in the denervated kidneys of SHRUDx (47 +/- 1 mmHg) compared with kidneys of the SHRS (50 +/- 1 mmHg; P < 0.04). Mean arterial pressure was approximately 12 mmHg lower in the bilaterally denervated SHRs than in the sham-operated SHRs.
Although bilateral denervation attenuated the development of hypertension in SHRs, unilateral denervation did not, indicating that one neurally intact kidney was sufficient to drive the normal development of SHR hypertension, but only with apparent prohypertensive compensatory changes in the denervated kidney.
确定慢性去神经支配对年轻成年自发性高血压大鼠（SHR）肾血管结构和功能的影响。
6周龄的SHR进行单侧肾去神经支配（SHRUDx）或假手术（SHRS）。10周时，将大鼠分配到旨在检查肾血管结构和功能的三种操作之一。另一组进行双侧肾去神经支配。
在SHRUDx或SHRS组中，肾脏要么进行灌注固定以进行动脉壁和管腔尺寸的体视学估计，要么进行血管铸型以确定小动脉管腔直径，要么将大鼠麻醉以估计肾小球毛细血管压力。
如先前报道，慢性单侧肾去神经支配对6至10周龄高血压的发展无显著影响，但导致小叶间动脉管腔狭窄（去神经支配组52±2μm，假手术组64±1μm；品系与处理之间的交互作用P<0.01），小叶间或弓形动脉壁尺寸无改变。对入球或出球小动脉管腔直径均无显著影响。与SHRS组的肾脏（50±1mmHg）相比，SHRUDx去神经支配肾脏的估计肾小球毛细血管压力显著降低（47±1mmHg；P<0.04）。双侧去神经支配的SHR的平均动脉压比假手术的SHR低约12mmHg。
虽然双侧去神经支配减弱了SHR高血压的发展，但单侧去神经支配则不然，这表明一个神经完整的肾脏足以驱动SHR高血压的正常发展，但仅在去神经支配的肾脏中伴有明显的促高血压代偿性变化。
