Kamata Kunio, Shinozaki Kuniko, Okada Mineyuki, Seto Yoshiyuki, Kobayashi Shinichi, Sakae Kenji, Oseto Mitsuaki, Natori Katsuro, Shirato-Horikoshi Haruko, Katayama Kazuhiko, Tanaka Tomoyuki, Takeda Naokazu, Taniguchi Koki
Technical Marketing Department, Denka-Seiken Co., Ltd., Gosen, Niigata, Japan.
J Med Virol. 2005 May;76(1):129-36. doi: 10.1002/jmv.20334.
Human noroviruses (NoVs), members of the genus Norovirus in the family Caliciviridae, are the leading agents of nonbacterial acute gastroenteritis worldwide. Human NoVs are currently divided into at least two genogroups, genogroup I (GI) and genogroup II (GII), each of which contains at least 14 and 17 genotypes. To explore the genetic and antigenic relationship among NoVs, we expressed the capsid protein of four genetically distinct NoVs, the GI/3 Kashiwa645 virus, the GII/3 Sanbu809 virus, the GII/5 Ichikawa754 virus, and the GII/7 Osaka10-25 virus in baculovirus expression system. An antigen enzyme-linked immunosorbent assay (ELISA) with hyperimmune serum against the four recombinant capsid proteins and characterized previously three capsid proteins derived from GI/1, GI/4, and GII/12 was developed to detect the NoVs antigen in stools. The antigen ELISA was highly specific to the homotypic strains, allowing assignment of a strain to a Norovirus genetic cluster within a genogroup.
人诺如病毒（NoVs）是杯状病毒科诺如病毒属的成员，是全球非细菌性急性胃肠炎的主要病原体。人诺如病毒目前至少分为两个基因组，基因组I（GI）和基因组II（GII），每个基因组分别包含至少14个和17个基因型。为了探究诺如病毒之间的遗传和抗原关系，我们在杆状病毒表达系统中表达了四种基因不同的诺如病毒的衣壳蛋白，即GI/3柏崎645病毒、GII/3三部809病毒、GII/5市川754病毒和GII/7大阪10 - 25病毒。我们开发了一种抗原酶联免疫吸附测定（ELISA），使用针对这四种重组衣壳蛋白的超免疫血清以及先前鉴定的源自GI/1、GI/4和GII/12的三种衣壳蛋白，用于检测粪便中的诺如病毒抗原。该抗原ELISA对同型菌株具有高度特异性，能够将一个菌株归属于一个基因组内的诺如病毒遗传簇。
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