Department of Virology II, National Institute of Infectious Diseases, Musashi-Murayama, Japan.
FEBS Open Bio. 2022 Sep;12(9):1688-1695. doi: 10.1002/2211-5463.13455. Epub 2022 Jul 4.
Noroviruses are major causative agents of nonbacterial acute gastroenteritis in humans. Ten genogroups of noroviruses have been identified to date, among which genogroup I (GI) and genogroup II (GII) noroviruses are major pathogens for humans. GI and GII noroviruses are further classified into nine and 27 genotypes, respectively. Noroviruses are well known to bind to histo-blood group antigens (HBGAs). Many studies have revealed that virus-like particles (VLPs) from different genotypes exhibit distinct patterns of HBGA binding, but the assay conditions used in these studies were not identical. To enable comparison of the binding to HBGA of nine GI genotypes, I purified VLPs from insect cells and analysed their HBGA-binding profiles. Although each genotype exhibited a distinct pattern of HBGA binding, Lewis b antigen was commonly recognized by all of the genogroup I strains, suggesting that this antigen plays a critical role in the pathogenesis of noroviruses.
诺如病毒是人类非细菌性急性胃肠炎的主要病原体。迄今为止，已鉴定出 10 种诺如病毒基因型，其中基因型 I（GI）和基因型 II（GII）诺如病毒是人类的主要病原体。GI 和 GII 诺如病毒进一步分为 9 种和 27 种基因型。众所周知，诺如病毒会与组织血型抗原（HBGAs）结合。许多研究表明，来自不同基因型的病毒样颗粒（VLPs）表现出不同的 HBGA 结合模式，但这些研究中使用的检测条件并不完全相同。为了能够比较 9 种 GI 基因型与 HBGA 的结合，我从昆虫细胞中纯化了 VLPs，并分析了它们的 HBGA 结合谱。尽管每种基因型都表现出独特的 HBGA 结合模式，但所有 GI 株都共同识别 Lewis b 抗原，这表明该抗原在诺如病毒的发病机制中发挥着关键作用。
