Voirin S G, Baumes R L, Sapis J C, Bayonove C L
INRA, Institut des Produits de la Vigne, Laboratoire des Arômes et des Substances Naturelles, Montpellier, France.
J Chromatogr. 1992 Mar 20;595(1-2):269-81. doi: 10.1016/0021-9673(92)85169-t.
Free and glycosidically bound terpenes of five Vitis vinifera grape cultivars (muscat of Alexandria, muscat of Frontignan, muscat of Hamburg, muscat Ottonel and Gewürztraminer) were investigated. The free and bound fractions were separated by selective retention on Amberlite XAD-2 resin. The glycosidic fractions were analysed by gas chromatography and gas chromatography-mass spectrometry using either enzymic hydrolysis and subsequent analysis of the released aglycones or trimethylsilyl (TMS) and trifluoroacetyl derivatives. The known monoterpenyl, benzyl and 2-phenylethyl beta-D-glucopyranosides, beta-rutinosides, 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranosides and 6-O-beta-D-apiofuranosyl-beta-D-glucopyranosides were determined. A number of other glycosides were detected and the structures of some of them, mainly apiosylglucosides and glucosides with aglycones in higher oxidation state than linalol, were tentatively identified using the mass spectra of their TMS and TFA derivatives and the results obtained from the analysis of their aglycones.
对五个葡萄品种（亚历山大麝香葡萄、弗龙蒂尼昂麝香葡萄、汉堡麝香葡萄、奥托奈麝香葡萄和琼瑶浆）中的游离萜烯和糖苷结合萜烯进行了研究。通过选择性保留在Amberlite XAD - 2树脂上分离游离和结合部分。糖苷部分通过气相色谱和气相色谱 - 质谱联用进行分析，使用酶促水解并随后分析释放的苷元，或三甲基硅烷基（TMS）和三氟乙酰衍生物。测定了已知的单萜基、苄基和2 - 苯乙基β - D - 葡萄糖苷、β - 芸香糖苷、6 - O - α - L - 阿拉伯呋喃糖基 - β - D - 葡萄糖苷和6 - O - β - D - 芹菜呋喃糖基 - β - D - 葡萄糖苷。检测到许多其他糖苷，并利用其TMS和TFA衍生物的质谱以及苷元分析结果初步鉴定了其中一些糖苷的结构，主要是芹菜糖基葡萄糖苷和苷元氧化态高于芳樟醇的葡萄糖苷。
