Tsui Kuan-Hao, Chen Ling-Yun, Shieh Miawh-Lirng, Chang Sheng-Ping, Yuan Chiou-Chung, Li Hsin-Yang
Department of Obstetrics and Gynecology, Veterans General Hospital, 201 Section 2, Shih-Pai Road, Taipei, Taiwan 112, Republic of China,
In Vitro Cell Dev Biol Anim. 2004 Nov-Dec;40(10):331-6. doi: 10.1290/0404033.1.
Precise paracrine cross-talk between the embryo and the endometrium is essential for the establishment of a successful pregnancy. Previous studies have demonstrated that the expression of interleukin-8 (IL-8) in the endometrium is enhanced during the late-secretory phase and early pregnancy. Furthermore, IL-8 receptor (IL-8R) expression has been detected in trophoblast cells of the developing embryo. To clarify the roles of IL-8 in the endometrium-embryo interactions, the effects of IL-8 on hormone secretion by trophoblast cells were studied using the BeWo trophoblast cell line that retains hormone-secreting properties of normal trophoblast cells. Using reverse transcription-polymerase chain reaction, we found that IL-8R messenger ribonucleic acid (mRNA) was expressed in BeWo cells. The levels of IL-8R mRNA and protein expression in BeWo cells were similar to those in primary first-trimester trophoblast cells. Progesterone (P4) secretion of BeWo cells was comparable with that of first-trimester trophoblast cells but higher than that of third-trimester trophoblast cells. Treatment of BeWo cells with recombinant human IL-8 (rhIL-8) had no effect on cell proliferation, as determined by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Interestingly, secretion of P4, but not human chorionic gonadotropin, from cultured BeWo cells was significantly enhanced when the cells were incubated with rhIL-8. Our results demonstrate that IL-8 may play an important role in the endometrium-embryo interactions by stimulating trophoblast secretion of P4 for maintenance of a successful pregnancy.
胚胎与子宫内膜之间精确的旁分泌串扰对于成功妊娠的建立至关重要。先前的研究表明，子宫内膜中白细胞介素-8（IL-8）的表达在分泌晚期和妊娠早期会增强。此外，在发育中胚胎的滋养层细胞中已检测到IL-8受体（IL-8R）的表达。为了阐明IL-8在子宫内膜-胚胎相互作用中的作用，使用保留正常滋养层细胞激素分泌特性的BeWo滋养层细胞系研究了IL-8对滋养层细胞激素分泌的影响。通过逆转录-聚合酶链反应，我们发现BeWo细胞中表达了IL-8R信使核糖核酸（mRNA）。BeWo细胞中IL-8R mRNA和蛋白表达水平与孕早期原代滋养层细胞中的水平相似。BeWo细胞的孕酮（P4）分泌与孕早期滋养层细胞相当，但高于孕晚期滋养层细胞。用重组人IL-8（rhIL-8）处理BeWo细胞对细胞增殖没有影响，这通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐测定法确定。有趣的是，当将培养的BeWo细胞与rhIL-8一起孵育时，P4的分泌（而非人绒毛膜促性腺激素）显著增强。我们的结果表明，IL-8可能通过刺激滋养层细胞分泌P4以维持成功妊娠，在子宫内膜-胚胎相互作用中发挥重要作用。
