Ren Yilong, Picout David R, Ellis Peter R, Ross-Murphy Simon B, Reid J S Grant
Department of Life Sciences, King's College London, Franklin-Wilkins Building, 150 Stamford Street, London SE1 9NH, UK.
Carbohydr Res. 2005 Apr 11;340(5):997-1005. doi: 10.1016/j.carres.2005.01.012.
This paper is the first multi-scale characterization of the xyloglucan extracted from seeds of the African tree Afzelia africana Se. Pers. It describes the extraction and characterization of this polysaccharide in terms of both primary monosaccharide and oligosaccharide composition. It also includes a study of the seed morphology. Morphological characterization includes optical, transmission, and scanning electron microscopy. The polysaccharide exists in thickened cell walls of the cotyledonary cells, and the extracted xyloglucan is structurally quite similar to those from tamarind seed and detarium. Nevertheless there are some subtle differences in the fine structure, particularly in the oligomeric xyloglucan composition. The chain flexibility of the polysaccharide is also discussed in the light of our recent measurements reported elsewhere [Biomacromolecules2004, 5, 2384-2391].
本文首次对从非洲树木非洲缅茄（Afzelia africana Se. Pers.）种子中提取的木葡聚糖进行了多尺度表征。它从初级单糖和寡糖组成方面描述了这种多糖的提取和表征。还包括对种子形态的研究。形态学表征包括光学显微镜、透射电子显微镜和扫描电子显微镜。该多糖存在于子叶细胞的加厚细胞壁中，提取的木葡聚糖在结构上与罗望子种子和猴面包树种子中的木葡聚糖非常相似。然而，在精细结构上存在一些细微差异，特别是在低聚木葡聚糖组成方面。还根据我们最近在其他地方报道的测量结果[《生物大分子》2004年，5卷，2384 - 2391页]讨论了该多糖的链柔韧性。
