Universidade Federal do Paraná, Departamento de Bioquímica e Biologia Molecular, CP 19046, CEP 81531-990 Curitiba, PR, Brazil.
Int J Biol Macromol. 2010 Mar 1;46(2):223-8. doi: 10.1016/j.ijbiomac.2009.12.012. Epub 2010 Jan 7.
Xyloglucans are water-soluble polysaccharides that serve as storage in cotyledonary tissues from seeds of Hymenaea courbaril among other Leguminosae. The use of xyloglucans is dependent on their solution properties. We now examine the influence of different times of extractions on its properties. Xyloglucans were obtained from milled and defatted cotyledons of H. courbaril by aqueous extraction at 25 degrees C for 0.25, 0.5, 1, 5, 15, 24, and 48 h. Composition data showed that increase in time lead to the increased protein contamination and arabinose content. Xyloglucans obtained with longer times of extraction had an average molar mass greater than those obtained with shorter times. The hydrodynamic radius and the radius of gyration also increased with the increase in the time of extraction. The ratio R(g)/R(h) was calculated and the values decreased slightly for increasing times of extraction. The effect of time also affected the viscosity which increased with the increase in time of extraction, and the longer ones probably contributing to the aggregation of xyloglucans.
木葡聚糖是一种水溶性多糖，作为 Hymenaea courbaril 等豆科植物子叶组织的储存物质。木葡聚糖的使用取决于其溶液性质。我们现在研究不同提取时间对其性质的影响。从 Hymenaea courbaril 的研磨和脱脂子叶中，通过在 25°C 下提取 0.25、0.5、1、5、15、24 和 48 小时，获得木葡聚糖。组成数据表明，时间的增加导致蛋白质污染和阿拉伯糖含量的增加。用较长时间提取得到的木葡聚糖的平均摩尔质量大于用较短时间提取得到的木葡聚糖。流体力学半径和回转半径也随提取时间的增加而增加。计算了 R(g)/R(h)的比值，并且随着提取时间的增加，该比值略有降低。时间的影响也影响了粘度，随着提取时间的增加而增加，而较长的时间可能导致木葡聚糖的聚集。
