Ranjit M R, Das A, Das B P, Das B N, Dash B P, Chhotray G P
Regional Medical Research Centre (ICMR), Po: S.E. Rly Complex, Chandrasekharpur, Bhubaneswar 751023, Orissa, India.
Trans R Soc Trop Med Hyg. 2005 May;99(5):389-95. doi: 10.1016/j.trstmh.2004.09.010.
A cross-sectional study was conducted in a malaria hyperendemic state of India to ascertain the distribution of Plasmodium falciparum genotypes in patients with mild (n=40) and severe (n=35) malaria. PCR and nested PCR were used to determine the glutamate-rich protein (GLURP), merozoite surface proteins 1 and 2 (MSP1 and MSP2) and knob-associated histidine-rich protein (KAHRP) for characterization of the parasite. The results indicate that (i) the 200bp allele of the MAD20 family of MSP1 and the 550bp allele of the 3D7 family of MSP2 show over-representation in severe malaria cases; (ii) the multiplicity of infection with respect to MSP2 alleles is significantly higher (P<0.001) in severe cases than in mild cases; and (iii) comparison with the findings of other studies leads to the conclusion that the distribution of P. falciparum genotypes between different clinical groups differs geographically.
在印度一个疟疾高度流行的邦开展了一项横断面研究，以确定轻度（n = 40）和重度（n = 35）疟疾患者中恶性疟原虫基因型的分布情况。采用聚合酶链反应（PCR）和巢式PCR来检测富含谷氨酸蛋白（GLURP）、裂殖子表面蛋白1和2（MSP1和MSP2）以及与小节相关的富含组氨酸蛋白（KAHRP），以对疟原虫进行特征分析。结果表明：（i）MSP1的MAD20家族的200bp等位基因和MSP2的3D7家族的550bp等位基因在重度疟疾病例中表现出超显性；（ii）重度病例中MSP2等位基因的感染多样性显著高于轻度病例（P<0.001）；（iii）与其他研究结果比较得出结论，不同临床组之间恶性疟原虫基因型的分布存在地理差异。
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