植物多酚毛蕊花糖苷对DNA的“体外”Fenton反应保护作用

"In vitro" protection of DNA from Fenton reaction by plant polyphenol verbascoside.

作者信息

Zhao Chenyang, Dodin Guy, Yuan Chenshan, Chen Haifeng, Zheng Rongliang, Jia Zhongjian, Fan Bo-Tao

机构信息

ITODYS-CNRS UMR 7086, University Paris 7-Denis Diderot, 1, rue Guy de la Brosse, 75005 Paris, France.

出版信息

Biochim Biophys Acta. 2005 May 25;1723(1-3):114-23. doi: 10.1016/j.bbagen.2005.02.004. Epub 2005 Mar 2.

DOI:10.1016/j.bbagen.2005.02.004

PMID:15780971

Abstract

The protection effect of verbascoside (Ver) against Fenton reaction on plasmid pBR322 DNA was studied using agarose gel electrophoresis and UV-visible spectroscopy. The pBR322 plasmid DNA is damaged by hydroxyl radical (OH*) generated from the Fenton reaction with H2O2 and Fe(II) or Fe(III). This DNA damage is characterized by the diminution of supercoiled DNA forms or by the increase of relaxed or linear DNA forms after oxidative attack. The UV spectrum study showed that verbascoside can form complexes with Fe(II) or Fe(III), and the complexation can be reversed by the addition of EDTA. The formation constants of verbascoside-Fe complexes were estimated as 10(21.03) and 10(31.94) M(-2) for Fe(II) and Fe(III) respectively. The inhibition of Fenton reaction by verbascoside could be partially explained by the sequestration of Fe ions.

摘要

采用琼脂糖凝胶电泳和紫外可见光谱法研究了毛蕊花糖苷（Ver）对芬顿反应损伤质粒pBR322 DNA的保护作用。pBR322质粒DNA会被由过氧化氢与亚铁离子或铁离子发生芬顿反应产生的羟基自由基（OH*）损伤。这种DNA损伤的特征是超螺旋DNA形式减少，或氧化攻击后松弛或线性DNA形式增加。紫外光谱研究表明，毛蕊花糖苷可与亚铁离子或铁离子形成络合物，加入乙二胺四乙酸（EDTA）可使络合作用逆转。毛蕊花糖苷-铁络合物的形成常数对于亚铁离子和铁离子分别估计为10（21.03）和10（31.94）M（-2）。毛蕊花糖苷对芬顿反应的抑制作用可部分归因于铁离子的螯合作用。