Guiral Marianne, Leroy Gisèle, Bianco Pierre, Gallice Philippe, Guigliarelli Bruno, Bruschi Mireille, Nitschke Wolfgang, Giudici-Orticoni Marie-Thérèse
Bioénergétique et Ingénierie des Protéines, CNRS, IBSM, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
Biochim Biophys Acta. 2005 May 25;1723(1-3):45-54. doi: 10.1016/j.bbagen.2005.01.015. Epub 2005 Feb 16.
The complex formation between the tetraheme cytochrome c3 and hexadecaheme high molecular weight cytochrome c (Hmc), the structure of which has recently been resolved, has been characterized by cross-linking experiments, EPR, electrochemistry and kinetic analysis, and some key parameters of the interaction were determined. The analysis of electron transfer between [Fe] hydrogenase, cytochrome c3 and Hmc demonstrates a redox-shuttling role of cytochrome c3 in the pathway from hydrogenase to Hmc, and shows an effect of redox state on the interaction between the two cytochromes. The role of polyheme cytochromes in electron transfer from periplasmic hydrogenase to membrane redox proteins is assessed. A model with cytochrome c3 as an intermediate between hydrogenase and various polyheme cytochromes is proposed and its physiological consequences are discussed.
四血红素细胞色素c3与十六血红素高分子量细胞色素c（Hmc）之间的复合物形成，其结构最近已得到解析，通过交联实验、电子顺磁共振、电化学和动力学分析对其进行了表征，并确定了相互作用的一些关键参数。对[Fe]氢化酶、细胞色素c3和Hmc之间电子转移的分析表明，细胞色素c3在从氢化酶到Hmc的途径中具有氧化还原穿梭作用，并显示出氧化还原状态对两种细胞色素之间相互作用的影响。评估了多血红素细胞色素在从周质氢化酶到膜氧化还原蛋白的电子转移中的作用。提出了以细胞色素c3作为氢化酶和各种多血红素细胞色素之间中间体的模型，并讨论了其生理后果。
