Randen I, Brown D, Thompson K M, Hughes-Jones N, Pascual V, Victor K, Capra J D, Førre O, Natvig J B
Institute of Immunology and Rheumatology, Oslo, Norway.
J Immunol. 1992 May 15;148(10):3296-301.
Using hybridoma technology we established a panel of human monoclonal rheumatoid factors (RF) from the synovial tissues of two patients with rheumatoid arthritis (RA), and one patient with polyarticular juvenile RA. Nucleotide sequence analysis of the V regions of these RF indicates that two independently derived antibodies from one of the RA patients are clonally related. One of these antibodies appears to be close to germ-line configuration, whereas the other has accumulated a total of 36 substitutions in both H and L chains. Measurements of the affinity for human IgG of the two RF show that the extensively mutated RF has 100-fold higher affinity for IgG than the RF close to germline. These findings indicate that IgM RF in RA can undergo affinity maturation and suggest that certain RF may be the product of an Ag-driven immune response.
利用杂交瘤技术，我们从两名类风湿性关节炎（RA）患者和一名多关节幼年型RA患者的滑膜组织中建立了一组人单克隆类风湿因子（RF）。对这些RF的V区进行核苷酸序列分析表明，来自其中一名RA患者的两种独立衍生抗体具有克隆相关性。其中一种抗体似乎接近种系构型，而另一种在重链和轻链中总共积累了36个替换。对这两种RF与人IgG亲和力的测量表明，广泛突变的RF对IgG的亲和力比对接近种系的RF高100倍。这些发现表明，RA中的IgM RF可以经历亲和力成熟，并提示某些RF可能是抗原驱动免疫反应的产物。
