希腊一组急性心肌炎致死病例心肌中的柯萨奇病毒B3序列
Coxsackievirus B3 sequences in the myocardium of fatal cases in a cluster of acute myocarditis in Greece.
作者信息
Spanakis N, Manolis E N, Tsakris A, Tsiodras S, Panagiotopoulos T, Saroglou G, Legakis N J
机构信息
Department of Microbiology, Medical School, University of Athens, 115 27 Athens, Greece.
出版信息
J Clin Pathol. 2005 Apr;58(4):357-60. doi: 10.1136/jcp.2004.020099.
AIM
The investigation of three fatal cases during a nationwide cluster of cases of an upper respiratory tract infection (URTI) associated with myocarditis and/or pericarditis in Greece in 2002.
METHODS
In the three women who died, necropsies were performed and tissue sections were taken for histological examination, antigen detection by immunohistochemistry and indirect immunofluorescence assay (IFA), amplification of viral genomes by nested reverse transcription polymerase chain reaction (RT-PCR), and sequence analysis.
RESULTS
All samples showed histological signs of active myocarditis. Immunohistochemistry revealed the presence of the enterovirus VP1 family of proteins and IFA revealed the presence of coxsackievirus B3 antigen. Nested RT-PCR amplified enteroviral alleles of the 5'-untranslated region which were identical to each other and to the coxsackievirus B3 sequences.
CONCLUSIONS
This study provides pathological evidence of enteroviral infection among fatal myocarditis cases in a nationwide URTI cluster of cases associated with myocarditis and/or pericarditis.
目的
调查2002年希腊全国范围内一起与心肌炎和/或心包炎相关的上呼吸道感染（URTI）聚集性病例中的三例死亡病例。
方法
对三名死亡女性进行尸检，并取组织切片进行组织学检查、通过免疫组织化学和间接免疫荧光试验（IFA）检测抗原、通过巢式逆转录聚合酶链反应（RT-PCR）扩增病毒基因组以及序列分析。
结果
所有样本均显示出活动性心肌炎的组织学迹象。免疫组织化学显示存在肠道病毒VP1蛋白家族，IFA显示存在柯萨奇病毒B3抗原。巢式RT-PCR扩增出5'-非翻译区的肠道病毒等位基因，这些等位基因彼此相同且与柯萨奇病毒B3序列相同。
结论
本研究为全国范围内与心肌炎和/或心包炎相关的URTI聚集性病例中致命心肌炎病例的肠道病毒感染提供了病理学证据。
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