Smets Koenraad, Keymeulen Annelies, Wollants Elke, Lagrou Katrien, Van Ranst Marc, Padalko Elizaveta
Department of Neonatology, Ghent University Hospital, De Pintelaan 185, B-9000 Gent, Belgium.
J Clin Virol. 2008 Jun;42(2):207-10. doi: 10.1016/j.jcv.2008.01.004. Epub 2008 Mar 7.
A fatal case of Coxsackie B3 myocarditis in a neonate is described. The clinical features became evident in the 3rd week of life, but enteroviral RNA was detected in the dried blood spot of the baby collected on day 4 after birth. This is the first report on the detection of enteroviral RNA in Guthrie card dried blood using reverse-transcriptase PCR. Materials and methods used are described in detail.
本文描述了一例新生儿柯萨奇B3型心肌炎致死病例。临床症状在出生后第3周变得明显，但在出生后第4天采集的婴儿干血斑中检测到肠道病毒RNA。这是首次关于使用逆转录聚合酶链反应在格思里卡片干血中检测肠道病毒RNA的报告。详细描述了所使用的材料和方法。
