Winstead Welby, Marshall Charles T, Lu Cheng L, Klueber Kathleen M, Roisen Fred J
Division of Otolaryngology-Head and Neck Surgery, University of Louisville, School of Medicine, Louisville, Kentucky 40202, USA.
Am J Rhinol. 2005 Jan-Feb;19(1):83-90.
The adult central nervous system contains progenitor cells; however, invasive surgery is required for their harvest. Olfactory neuroepithelium (ONe) has attracted attention because it is extracranial and contains progenitor cells that account for its regenerative capacity. Olfactory progenitor cells have been cultured from postmortem ONe. Our aim was to determine if olfactory progenitors could be obtained via biopsy from patients in a feasible, effective, and safe manner.
Endoscopic biopsy was performed on individuals undergoing sinus surgery (n = 42). Olfactory function was assessed pre- and postoperatively. Specimens were cultured under conditions for olfactory progenitor cell development.
Progenitor cells emerged in cultures from 50% of our patients. The superior turbinate, biopsied with cutting punch forceps, gave the highest yield. No adverse impact on olfaction or complications with the biopsy were observed.
Endoscopic biopsy of ONe for obtaining olfactory progenitor cells from living donors is feasible, effective, and safe.
成体中枢神经系统含有祖细胞；然而，获取这些祖细胞需要进行侵入性手术。嗅神经上皮（ONe）因其位于颅外且含有具有再生能力的祖细胞而受到关注。嗅祖细胞已从死后的嗅神经上皮中培养出来。我们的目的是确定能否以可行、有效且安全的方式通过活检从患者体内获取嗅祖细胞。
对接受鼻窦手术的个体（n = 42）进行内镜活检。术前和术后评估嗅觉功能。将标本在嗅祖细胞发育的条件下进行培养。
我们50%的患者培养物中出现了祖细胞。用切割活检钳取材的上鼻甲产生的细胞产量最高。未观察到活检对嗅觉有不利影响或出现并发症。
通过内镜活检嗅神经上皮从活体供体获取嗅祖细胞是可行、有效且安全的。
Zotero 插件