使用纳米吸管和纳米颗粒进行单DNA分子检测。

Single DNA molecule detection using nanopipettes and nanoparticles.

作者信息

Karhanek Miloslav, Kemp Jennifer T, Pourmand Nader, Davis Ronald W, Webb Chris D

机构信息

Department of Biochemistry, Stanford Genome Technology Center, Stanford University, Stanford, CA 94304, USA.

出版信息

Nano Lett. 2005 Feb;5(2):403-7. doi: 10.1021/nl0480464.

DOI:10.1021/nl0480464

PMID:15794633

Abstract

Single DNA molecules labeled with nanoparticles can be detected by blockades of ionic current as they are translocated through a nanopipette tip formed by a pulled glass capillary. The nanopipette detection technique can provide not only tools for detection and identification of single DNA and protein molecules but also deeper insight and understanding of stochastic interactions of various biomolecules with their environment.

摘要

当用纳米颗粒标记的单个DNA分子穿过由拉制的玻璃毛细管形成的纳米移液器尖端时，可以通过离子电流的阻断来检测它们。纳米移液器检测技术不仅可以提供检测和识别单个DNA和蛋白质分子的工具，还可以更深入地洞察和理解各种生物分子与其环境的随机相互作用。