Pumera Martin, Smíd Bretislav
ICYS, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, Japan.
J Nanosci Nanotechnol. 2007 Oct;7(10):3590-5. doi: 10.1166/jnn.2007.846.
Double wall carbon nanotubes are noncovalently functionalized with redox protein and such assembly is used for construction of electrochemical binder-less glucose biosensor. Redox protein glucose oxidase performs as biorecognition element and double wall carbon nanotubes act both as immobilization platform for redox enzyme and as signal transducer. The double carbon nanotubes are characterized by cyclic voltammetry and specific surface area measurements; the redox protein noncovalently functionalized double wall carbon nanotubes are characterized in detail by X-ray photoelectron spectroscopy, cyclic voltammetry, amperometry, and transmission electron microscopy.
双壁碳纳米管通过氧化还原蛋白进行非共价功能化，这种组装用于构建无电化学粘合剂的葡萄糖生物传感器。氧化还原蛋白葡萄糖氧化酶作为生物识别元件，双壁碳纳米管既作为氧化还原酶的固定平台，又作为信号转导器。通过循环伏安法和比表面积测量对双壁碳纳米管进行表征；通过X射线光电子能谱、循环伏安法、安培法和透射电子显微镜对非共价功能化的氧化还原蛋白双壁碳纳米管进行详细表征。
