白藜芦醇对PC12细胞中氧化型低密度脂蛋白细胞毒性的神经保护作用

[Neuroprotection by resveratrol against cytotoxicity of oxidized low density lipoprotein in PC12 cells].

作者信息

Cao Li, Wang Yong-jun

机构信息

Department of Neurology, Tiantan Hospital, Capital University of Medical Sciences, Beijing 100050, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2005 Jan;39(1):19-21.

PMID:15794885

Abstract

OBJECTIVE

To investigate neuroprotection of resveratrol against cytotoxicity of oxidized low density lipoprotein (oxLDL) in PC12 cells.

METHODS

PC12 cells were pretreated with resveratrol for one hour as a neuron model and then exposed to oxLDL at varied concentrations. Effects of resveratrol on cell viability, permeability of cell membrane, cell nucleus and activity of caspase-3 were evaluated with MTT assay, lactic acid dehydrogenase (LDH) release assay, DNA fragmentation (TUNEL) assay and assay for caspase-3 activity (caspase assay).

RESULTS

Cell viability, LDH release rate, percentage of cells with positive TUNEL and activity of caspase-3 were (62 +/- 3)%, (23 +/- 3)%, (26 +/- 5)% and (0.811 +/- 0.049) mol.min(-1).microg(-1), vs (84 +/- 7)%, (13 +/- 4)%, (12 +/- 4)% and (0.553 +/- 0.048) mol.min(-1).microg(-1) in PC12 cells treated with 10 mg/L oxLDL vs in those treated with 10 mg/L oxLDL plus 50 micromol/L resveratrol, respectively, with statistically significant difference.

CONCLUSION

Resveratrol could attenuate cytotoxicity induced by oxLDL in PC12 cells with neuroprotection.

摘要

目的

研究白藜芦醇对氧化型低密度脂蛋白（oxLDL）诱导的PC12细胞毒性的神经保护作用。

方法

将PC12细胞作为神经元模型，用白藜芦醇预处理1小时，然后暴露于不同浓度的oxLDL中。采用MTT法、乳酸脱氢酶（LDH）释放法、DNA片段化（TUNEL）法和半胱天冬酶-3活性检测法（caspase检测法）评估白藜芦醇对细胞活力、细胞膜通透性、细胞核及半胱天冬酶-3活性的影响。

结果

在10 mg/L oxLDL处理的PC12细胞中，细胞活力、LDH释放率、TUNEL阳性细胞百分比和半胱天冬酶-3活性分别为（62±3）%、（23±3）%、（26±5）%和（0.811±0.049）mol·min⁻¹·μg⁻¹；而在10 mg/L oxLDL加50 μmol/L白藜芦醇处理的细胞中，上述指标分别为（84±7）%、（13±4）%、（12±4）%和（0.553±0.048）mol·min⁻¹·μg⁻¹，差异有统计学意义。