通过魔角旋转1H核磁共振波谱和模式识别研究硝酸镧对肝脏和肾脏组织的急性生化效应

Acute biochemical effects of La(NO3)3 on liver and kidney tissues by magic-angle spinning 1H nuclear magnetic resonance spectroscopy and pattern recognition.

作者信息

Wu Huifeng, Zhang Xiaoyu, Li Xiaojing, Wu Yijie, Pei Fengkui

机构信息

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.

出版信息

Anal Biochem. 2005 Apr 15;339(2):242-8. doi: 10.1016/j.ab.2005.01.021.

DOI:10.1016/j.ab.2005.01.021

PMID:15797564

Abstract

High-resolution magic-angle spinning (MAS) 1H nuclear magnetic resonance (NMR) spectroscopic and pattern recognition (PR) based methods have been applied to studies on the acute biochemical effects of La(NO3)3 on rats. Male Wistar rats were treated with various doses of La(NO3)3 (2, 10, and 50 mg/kg body weight), and MAS 1H NMR spectra of their intact liver and kidney tissues were analyzed using principal components analysis to extract metabolic information. The biochemical effects of La(NO3)3 were characterized by the increase of triglyceride and bile acid and the decrease of glycogen in liver tissue, together with a slight elevation of triglyceride level in kidney tissue. The target lesion of La(NO3)3 to liver was found by MAS NMR-PR methods. This study illustrated the power of the combination of MAS 1H NMR and pattern recognition for the analysis of biochemical effects of rare earths.

摘要

基于高分辨率魔角旋转（MAS）1H核磁共振（NMR）光谱和模式识别（PR）的方法已应用于研究硝酸镧对大鼠的急性生化效应。雄性Wistar大鼠接受不同剂量的硝酸镧（2、10和50mg/kg体重）处理，使用主成分分析对其完整肝脏和肾脏组织的MAS 1H NMR光谱进行分析，以提取代谢信息。硝酸镧的生化效应表现为肝脏组织中甘油三酯和胆汁酸增加、糖原减少，同时肾脏组织中甘油三酯水平略有升高。通过MAS NMR-PR方法发现了硝酸镧对肝脏的靶病变。本研究说明了MAS 1H NMR与模式识别相结合在分析稀土生化效应方面的作用。