Kim Seo Jin, Jung Hak Hyun
Department of Biomedical Sciences--Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea.
Acta Otolaryngol. 2005 Jan;125(1):43-7. doi: 10.1080/00016480410018151.
These results suggest that CCSP is upregulated in OME and may play a protective role in the pathogenesis of OME.
Clara cell secretory protein (CCSP) is an abundant 16-kDa homodimeric protein and is secreted by non-ciliated secretory epithelial cells in the lung. It has an important protective role against the intrapulmonary inflammatory process. The aim of this study was to investigate the expression of CCSP in endotoxin-induced otitis media with effusion (OME) in the rat.
We instilled endotoxin and saline (control) into the middle ear cavity of the rat. Middle ear mucosa were taken at 0, 1, 3, 6 and 12 h and 1, 3, 7 and 14 days, and the expression of both CCSP mRNA and protein were then evaluated using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry.
RT-PCR revealed that the expression of CCSP was first identified at 1 h after endotoxin instillation, was dramatically increased between 1 h and Day 1, with maximal expression at 12 h, and then decreased after Day 3. The expression pattern of CCSP protein identified by means of Western blotting was similar to the CCSP mRNA patterns observed using RT-PCR. Expression of CCSP at both mRNA and protein levels was not detected in either normal middle ear mucosa or saline-instilled middle ear mucosa. Immunohistochemistry revealed that some epithelial cells in the middle ear mucosa were stained.
这些结果表明，克拉拉细胞分泌蛋白（CCSP）在中耳积液（OME）中表达上调，可能在OME的发病机制中发挥保护作用。
克拉拉细胞分泌蛋白（CCSP）是一种丰富的16 kDa同二聚体蛋白，由肺内无纤毛的分泌上皮细胞分泌。它对肺内炎症过程具有重要的保护作用。本研究旨在探讨CCSP在大鼠内毒素诱导的中耳积液（OME）中的表达。
我们将内毒素和生理盐水（对照）注入大鼠中耳腔。在0、1、3、6和12小时以及1、3、7和14天采集中耳黏膜，然后使用半定量逆转录聚合酶链反应（RT-PCR）、蛋白质印迹法和免疫组织化学法评估CCSP mRNA和蛋白的表达。
RT-PCR显示，内毒素注入后1小时首次检测到CCSP的表达，在1小时至第1天之间显著增加，在12小时达到最大表达，然后在第3天后下降。通过蛋白质印迹法确定的CCSP蛋白表达模式与使用RT-PCR观察到的CCSP mRNA模式相似。在正常中耳黏膜或注入生理盐水的中耳黏膜中均未检测到CCSP在mRNA和蛋白水平的表达。免疫组织化学显示中耳黏膜中的一些上皮细胞被染色。
