Norberg Mikael, Holmlund Mattias, Nilsson Ove
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-90183 Umeå, Sweden.
Development. 2005 May;132(9):2203-13. doi: 10.1242/dev.01815. Epub 2005 Mar 30.
Developmental processes in multicellular organisms involve an intricate balance between mechanisms that promote cell division activity and growth, and others that promote cell differentiation. Leaf development in Arabidopsis thaliana is controlled by genes like BLADE ON PETIOLE1 (BOP1), which prevent the development of ectopic meristematic activity that leads to the formation of new organs, and JAGGED (JAG), which control the proximodistal development of the leaf by regulating cell-division activity. We have isolated and characterized the BOP1 gene together with a functionally redundant close homolog that we name BOP2. The BOP genes are members of a gene family containing ankyrin repeats and a BTB/POZ domain, suggesting a role in protein-protein interaction. We show that the BOP genes are expressed in the proximal parts of plant lateral organs where they repress the transcription not only of class 1 knox genes but also of JAG. We also show that the BOP genes are acting together with the flower meristem identity gene LEAFY in the suppression of bract formation. These findings show that the BOP genes are important regulators of the growth and development of lateral organs.
多细胞生物体中的发育过程涉及促进细胞分裂活性和生长的机制与促进细胞分化的机制之间的复杂平衡。拟南芥的叶片发育受诸如叶柄上的叶片1（BOP1）等基因控制，这些基因可防止导致新器官形成的异位分生组织活性的发育，以及锯齿状（JAG）基因，其通过调节细胞分裂活性来控制叶片的近远轴发育。我们已经分离并鉴定了BOP1基因以及一个功能冗余的紧密同源基因，我们将其命名为BOP2。BOP基因是一个包含锚蛋白重复序列和BTB/POZ结构域的基因家族的成员，表明其在蛋白质-蛋白质相互作用中发挥作用。我们发现BOP基因在植物侧生器官的近端部分表达，在那里它们不仅抑制1类knox基因的转录，还抑制JAG的转录。我们还表明，BOP基因与花分生组织特性基因叶状（LEAFY）共同作用来抑制苞片的形成。这些发现表明BOP基因是侧生器官生长和发育的重要调节因子。
