Brumbt Agnès, Ravelet Corinne, Grosset Catherine, Ravel Anne, Villet Annick, Peyrin Eric
Département de Pharmacochimie Moléculaire UMR 5063 CNRS, Institut de Chimie Moléculaire de Grenoble FR 2607, Université Joseph Fourier, UFR de Pharmacie de Grenoble, Avenue de Verdun, 38240 Meylan, France.
Anal Chem. 2005 Apr 1;77(7):1993-8. doi: 10.1021/ac048344l.
An immobilized anti-L-arginine d-RNA aptamer, used as a target-specific chiral stationary phase (CSP), was found to be very quickly degraded by RNases under usual chromatographic utilization and storage. To overcome this severe limitation for a practical use, a CSP based on the L-RNA aptamer, that is, the mirror image of the D-RNA aptamer, was created. It was shown that this mirror-image approach was a very simple and powerful strategy to develop a highly stable stationary phase due to the intrinsic insensitivity of l-RNA to the RNase degradation. In addition, such an approach allowed one to reverse the enantiomer elution order relative to that obtained with the corresponding d-RNA CSP.
一种固定化的抗L-精氨酸d-RNA适配体用作目标特异性手性固定相（CSP），发现在常规色谱使用和储存条件下会被核糖核酸酶迅速降解。为克服这一实际应用中的严重限制，构建了一种基于L-RNA适配体（即D-RNA适配体的镜像）的CSP。结果表明，这种镜像方法是开发高度稳定固定相的一种非常简单且有效的策略，因为L-RNA对核糖核酸酶降解具有内在的不敏感性。此外，这种方法能够使对映体洗脱顺序相对于相应的d-RNA CSP得到的洗脱顺序发生反转。
