Department of Chemistry, University of Illinois at Urbana-Champaign Urbana, IL 61801, USA.
Analyst. 2010 Feb;135(2):419-22. doi: 10.1039/b921267a. Epub 2009 Dec 22.
The natural chirality of DNA and RNA aptamers has been used to develop fluorescent agents to determine the enantiomeric ratio of adenosine and arginine, respectively. The quantification is based on structure-switching DNA or RNA aptamers labeled with fluorophore and quencher, allowing chiral detection down to 0.1 : 99.9 (L : D) for arginine after calibration. Such a method provides a general platform for simple, low-cost and high throughput detection and quantification of chirality of a broad range of molecules.
DNA 和 RNA 适体的天然手性已被用于开发荧光试剂，分别用于测定腺苷和精氨酸的对映体比例。定量基于标记有荧光团和猝灭剂的结构切换 DNA 或 RNA 适体，经校准后，可将精氨酸的手性检测下限降低至 0.1 : 99.9（L : D）。该方法为广泛分子的手性的简单、低成本和高通量检测和定量提供了通用平台。
