Di Bernardo G, Galderisi U, Cipollaro M, Cascino A
Department of Experimental Medicine, Section of Biotechnologies and Molecular Biology, 2nd University of Naples, Via Costantinopoli 16, 80138 Naples, Italy.
Biotechnol Prog. 2005 Mar-Apr;21(2):546-9. doi: 10.1021/bp049710p.
We describe here a molecular method that can be used to detect genome traits of a given horticultural item at each stage from the farm to the market. We developed a procedure to extract and amplify by PCR DNA obtained from complex matrixes, such as dried figs and fig jam. Few fragmented DNA molecules can be recovered from food products. However, we were able to increase the yield of PCR reactions by successfully applying an enzymatic repair protocol to retrieved DNA.
我们在此描述一种分子方法，该方法可用于检测从农场到市场的每个阶段给定园艺产品的基因组特征。我们开发了一种程序，用于从复杂基质（如干无花果和无花果果酱）中提取DNA并通过PCR进行扩增。从食品中只能回收很少的片段化DNA分子。然而，我们通过成功地将酶修复方案应用于回收的DNA，提高了PCR反应的产量。
