Doveri Silvia, Lee David
Crop Genetics Group, NIAB, Huntingdon Road, Cambridge CB3 0LE, United Kingdom.
J Agric Food Chem. 2007 Jun 13;55(12):4640-4. doi: 10.1021/jf063259v. Epub 2007 May 19.
The 5S intergenic spacers were amplified using a common pair of primers and sequenced from four species (Brassica napus, Zea mays, Helianthus annuus, and Glycine max). Crop-specific assays were developed from primers designed from the spacers and tested to amplify corresponding DNAs in both conventional end-point and real-time polymerase chain reactions (PCRs). The high copy numbers of the 5S DNA in plants make it possible to detect very small amounts of DNA using this marker. This sensitivity made it possible to compare different DNA extraction methods for highly processed food products using 5S spacers, even allowing dilution of templates to overcome PCR inhibition.
使用一对通用引物扩增5S基因间隔区，并对四个物种（甘蓝型油菜、玉米、向日葵和大豆）进行测序。根据间隔区设计引物，开发了针对特定作物的检测方法，并在传统终点聚合酶链反应（PCR）和实时PCR中进行测试，以扩增相应的DNA。植物中5S DNA的高拷贝数使得使用该标记能够检测到极少量的DNA。这种灵敏度使得使用5S间隔区比较高度加工食品的不同DNA提取方法成为可能，甚至可以稀释模板以克服PCR抑制。
