Holman Alexander G, Coffin John M
Department of Molecular Microbiology, Sackler School of Graduate Biomedical Sciences, Tufts University School of Medicine, Boston, MA 02111, USA.
Proc Natl Acad Sci U S A. 2005 Apr 26;102(17):6103-7. doi: 10.1073/pnas.0501646102. Epub 2005 Mar 31.
To investigate retroviral integration targeting on a nucleotide scale, we examined the base frequencies directly surrounding cloned in vivo HIV-1, murine leukemia virus, and avian sarcoma/leukosis virus integrations. Base preferences of up to 2-fold the expected frequencies were found for three viruses, representing P values down to <10(-100) and defining what appear to be preferred integration sequences. Offset symmetry reflecting the topology of the integration reaction was found for HIV-1 and avian sarcoma/leukosis virus but not murine leukemia virus, suggesting fundamental differences in the way different retroviral integration complexes interact with host-cell DNA.
为了在核苷酸水平上研究逆转录病毒整合靶向，我们直接检查了克隆的体内HIV-1、鼠白血病病毒和禽肉瘤/白血病病毒整合位点周围的碱基频率。发现三种病毒的碱基偏好高达预期频率的2倍，P值低至<10(-100)，并确定了似乎是偏好的整合序列。在HIV-1和禽肉瘤/白血病病毒中发现了反映整合反应拓扑结构的偏移对称性，但在鼠白血病病毒中未发现，这表明不同逆转录病毒整合复合物与宿主细胞DNA相互作用的方式存在根本差异。
