Morphological recovery of the granule cells from the olfactory bulb after the cessation of acute ozone exposure.

The purpose of this study was to analyze the possible morphological recovery of the granule cells in the olfactory bulb as a consequence of oxidative stress after an acute ozone exposure. Rats were divided in two groups: Control (air exposed) and experimental group, exposed 4 h, to 1 ppm ozone and divided into 4 subgroups, which were sacrificed at 2 and 24 h, 10 and 15 days, respectively. Olfactory bulbs were processed with the rapid Golgi method and for transmission electron microscopy. The granule cells of the olfactory bulb disclosed less dendritic spine density at 2, 24 h, and 10 days after the exposure compared with controls. At 15 days, the number of spines increased to values similar to those found in controls. The granule cells ultrastructure demonstrated an increment in lipofucsin granules, as well as swollen organelles, changes that decreased overtime. This change decline might be related to a partial recovery of the associative granule cells function.