Villalobos Virginia, Bonilla Ernesto, Castellano Alan, Novo Ernesto, Caspersen Ralph, Giraldoth Debora, Medina-Leendertz Shirley
Departamento de Biología, Facultad Experimental de Ciencias, Universidad del Zulia, Maracaibo, Venezuela.
Biocell. 2009 Dec;33(3):187-97.
The effect of manganese toxicity on the ultrastructure of the olfactory bulb was evaluated. Male albino mice were injected intraperitoneally with MnCl2 (5 mg/Kg/day) five days per week during nine weeks. The control group received NaCl (0.9%). The olfactory bulbs of five mice from each group were processed for transmission electron microscopy after 2, 4, 6 and 9 weeks of manganese treatment. On week 2, some disorganization of the myelin sheaths was observed. After 4 weeks, degenerated neurons with dilated cisternae of rough endoplasmic reticulum and swollen mitochondria appeared. A certain degree of gliosis with a predominance of astrocytes with swollen mitochondria, disorganization of the endomembrane system, dilation of the perinuclear cisternae and irregularly shaped nuclei with abnormal chromatin distribution were observed after 6 weeks. Some glial cells showed disorganization of the Golgi apparatus. On week 9, an increase in the number of astrocytes, whose mitochondrial cristae were partially or totally erased, and a dilation of the rough endoplasmic reticulum were found. Neurons appear degenerated, with swollen mitochondria and a vacuolated, electron dense cytoplasm. These changes seem to indicate that the olfactory bulb is sensitive to the toxic effects of manganese.
评估了锰毒性对嗅球超微结构的影响。雄性白化小鼠每周五天腹腔注射MnCl₂（5毫克/千克/天），持续九周。对照组接受NaCl（0.9%）。在锰处理2、4、6和9周后，对每组五只小鼠的嗅球进行透射电子显微镜检查。在第2周时，观察到髓鞘有一些紊乱。4周后，出现了内质网池扩张和线粒体肿胀的退化神经元。6周后，观察到一定程度的胶质细胞增生，以线粒体肿胀的星形胶质细胞为主，内膜系统紊乱，核周池扩张，核形状不规则，染色质分布异常。一些神经胶质细胞显示高尔基体紊乱。在第9周时，发现星形胶质细胞数量增加，其线粒体嵴部分或完全消失，粗面内质网扩张。神经元出现退化，线粒体肿胀，细胞质空泡化且电子密度增加。这些变化似乎表明嗅球对锰的毒性作用敏感。
