Huang H T, Lin H S, Lu K S
J Neural Transm. 1979;45(3):253-64. doi: 10.1007/BF01244413.
Nerve terminals in the pineal glands of control, parachlorophenylalanine (PCPA)-, reserpine- and nialamide-administered hamsters were studied with glutaraldehyde-dichromate (GD) and formaldehyde-glutaraldehyde-dichromate (FGD) techniques in the electron microscope. In control pineals treated with the FGD or GD technique, clusters of electron dense reactive particles with a diameter of 20-30 nm, and mainly located in the perivascular space, were identified as dense cores of the small granulated vesicles (SGVs) in the pineal nerve fibers. After the administration of PCPA, the reactive cores were greatly decreased in number in the FGD-treated pineal blocks, whereas a lot of clusters of reactive cores were observed in the perivascular space in GD-treated specimens. After reserpine treatment, no reactive structures were revealed by GD or FGD technique. Reactive cores appeared to be increased in number and in electron density in GD- or FGD-treated pineals of hamsters after administration of nialamide. From the observations, it is concluded that the dense cores of SGVs in the pineal nerve fibers contain both primary catecholamines and 5-hydroxytryptamine although it was not determined whether both amines are located in the same or separate vesicles.
运用戊二醛 - 重铬酸盐（GD）和甲醛 - 戊二醛 - 重铬酸盐（FGD）技术，在电子显微镜下研究了对照组、对氯苯丙氨酸（PCPA）处理组、利血平处理组和尼亚酰胺处理组仓鼠松果体中的神经末梢。在用FGD或GD技术处理的对照松果体中，直径为20 - 30纳米、主要位于血管周围间隙的电子致密反应性颗粒簇，被鉴定为松果体神经纤维中小颗粒囊泡（SGV）的致密核心。给予PCPA后，在FGD处理的松果体块中，反应性核心的数量大幅减少，而在GD处理的标本中，在血管周围间隙观察到许多反应性核心簇。利血平处理后，GD或FGD技术均未显示出反应性结构。给予尼亚酰胺后，在GD或FGD处理的仓鼠松果体中，反应性核心的数量和电子密度似乎增加。从这些观察结果得出结论，松果体神经纤维中SGV的致密核心既含有原发性儿茶酚胺又含有5 - 羟色胺，尽管尚未确定这两种胺是位于同一囊泡还是分开的囊泡中。
