[Design of laser-induced fluorescence detector for high performance liquid chromatography].

Based on confocal configuration, a laser-induced fluorescence detector (LIFD) for high performance liquid chromatography (HPLC) is described and evaluated. A diode double-pumped solid-state laser emitting at 473 nm is used as excitation source. A detection cell of 7 microL physical volume and 0.1 nL effective optical volume was designed for conventional HPLC-LIFD. The angle between entrance and outlet channels of the cell was 120 degrees, without any change of internal diameters or right-angles along the channels. These measures insured the elimination of bubble accumulation or formation inside the cell, and additional band broadening effect. Riboflavin standard was used as test sample to evaluate the performance of the HPLC-LIFD. The analyses of real samples containing riboflavin were demonstrated.