发光二极管诱导化学发光及其在高效液相色谱中的应用。
Light emitting diode induced chemiluminescence and its application as a detector for high performance liquid chromatography.
机构信息
Key Lab of Green Chemistry & Technology of MOE, College of Chemistry, Sichuan University, Chengdu, Sichuan 610064, China.
出版信息
J Chromatogr A. 2009 Dec 18;1216(51):8926-32. doi: 10.1016/j.chroma.2009.10.060. Epub 2009 Oct 30.
Some categories of compounds, including quinones, coumarins, flavins, and xanthene dyes, were found to produce strong chemiluminescence (CL) signals with luminol in sample solution under the irradiation of light emitting diodes (LED) with proper wavelengths. Based on this phenomenon, a compact photochemical reactor was constructed to develop a novel LED induced CL detector for high performance liquid chromatography (HPLC). The effects of related parameters including LED wavelength, luminol concentration, flow rate, pH, and eluents of HPLC were investigated in detail. Under the optimized conditions, the limits of detections (LODs) were in the range of 0.2-80 ng mL(-1). The applications and accuracy of the proposed method were validated by analyzing food samples such as milk powder, beer, candy and beverage with satisfactory results.
一些化合物类别，包括醌类、香豆素类、黄素类和呫吨染料，在适当波长的发光二极管（LED）照射下，在样品溶液中与鲁米诺一起产生强化学发光（CL）信号。基于这一现象，构建了一个紧凑型光化学反应器，以开发用于高效液相色谱（HPLC）的新型 LED 诱导 CL 检测器。详细研究了相关参数的影响，包括 LED 波长、鲁米诺浓度、流速、pH 值和 HPLC 的洗脱液。在优化条件下，检测限（LOD）范围为 0.2-80ngmL(-1)。通过对奶粉、啤酒、糖果和饮料等食品样品进行分析，验证了该方法的应用和准确性，结果令人满意。
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