Hu Li, Yang Xiupei, Wang Chunling, Yuan Hongyan, Xiao Dan
College of Chemistry, Sichuan University, Chengdu 610065, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Sep 1;856(1-2):245-51. doi: 10.1016/j.jchromb.2007.06.011. Epub 2007 Jun 22.
A simple, rapid and sensitive method was developed for routine analysis of riboflavin in beverage, green tea and urine by capillary electrophoresis with in-column optical fiber laser-induced fluorescence detection (LIF). The difference between the present detector in the study and others is that an optical fiber was adopted in the former, which can guide the excitation light into the capillary right at the detection window. The linearity of the method (r(2)=0.998) was good over the concentration range from 0.05 to 20 microM for riboflavin. The limit of detection (LOD) was determined using linear regression analysis and was found to be 3.0 nM. The percent recoveries of riboflavin in beverage, green tea and urine samples were 95.3+/-2.9, 105.5+/-3.9 and 94.3+/-1.7, respectively. These results of quantitative analysis of riboflavin in beverage and green tea samples is in agreement with that of obtained by the AOAC of fluorometric method. In the analysis of urine samples, all electropherograms of urine samples and corresponding concentrations of riboflavin in the period of 13 h after orally administrating the ingestion of vitamin B(2) tablets were illustrated.
建立了一种简单、快速且灵敏的方法，用于通过柱内光纤激光诱导荧光检测（LIF）的毛细管电泳对饮料、绿茶和尿液中的核黄素进行常规分析。本研究中的检测器与其他检测器的不同之处在于，前者采用了光纤，可将激发光直接引导至检测窗口处的毛细管中。该方法对于核黄素在0.05至20 microM的浓度范围内线性良好（r(2)=0.998）。使用线性回归分析确定检测限（LOD），结果为3.0 nM。饮料、绿茶和尿液样品中核黄素的回收率分别为95.3±2.9、105.5±3.9和94.3±1.7。饮料和绿茶样品中核黄素定量分析的这些结果与通过AOAC荧光法获得的结果一致。在尿液样品分析中，展示了口服维生素B(2)片后13小时内尿液样品的所有电泳图以及相应的核黄素浓度。
